Anti-bFGF antibody

Anti-bFGF antibody

• 种属反应性Human,Mouse,Rat,Chicken,Cow,Rabbit,Sheep

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human bFGF 143-250/288

• 偶联Non-conjugated

• Anti-bFGF antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Secreted. Nucleus.

• 其它名称Basic fibroblast growth factor
  FGF basic
  FGF-basic
  BFGF
  FGF-2
  FGF B
  FGF2
  FGF2 basic
  FGFB
  Fibroblast growth factor 2 (basic)
  HBGF 2
  HBGF-2
  HBGF2
  HBGH 2
  HBGH2
  Heparin binding growth factor 2 precursor
  Prostatropin
  FGF2_HUMAN.

  more

• Anti-bFGF antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

• 

  Fig1: Blank control (Black line): Molt4 (Black).
  Primary Antibody (green line): Rabbit Anti-bFGF antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  

  Fig2: Sample:
  HepG2(Human) Cell Lysate at 30 ug
  Primary: Anti-bFGF  at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 18 kD
  Observed band size: 18 kD
  

  

  Fig3: Tissue/cell: human cervical cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-bFGF Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Sample: Spleen (Mouse) Lysate at 40 ug
  Primary: Anti- bFGF at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 17 kD
  Observed band size: 18 kD

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