Anti-B4GALNT1/GM2 synthase antibody

Anti-B4GALNT1/GM2 synthase antibody

• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Rabbit,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human B4GALNT1/GM2 synthase 451-533/533

• 偶联Non-conjugated

• Anti-B4GALNT1/GM2 synthase antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A.

• 亚细胞定位Golgi apparatus membrane; Single-pass type II membrane protein.

• 其它名称(N acetylneuraminyl) galactosylglucosylceramide
  (N-acetylneuraminyl)-galactosylglucosylceramide
  4 N-acetylgalactosaminyltransferase 1
  4933429D13Rik
  B4GALNT1
  B4GN1_HUMAN
  Beta 1 4 N acetyl galactosaminyl transferase 1
  Beta 1 4 N acetylgalactosaminyltransferase 1
  Beta-1
  BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE 1
  BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE
  Beta1 4GalNAc T
  Gal-NAc-T
  GALGT
  Galgt1
  GalNAc T
  GalNAc-T
  GALNACT
  GD2 synthase
  Ggm-2
  Ggm2
  GM2 synthase
  GM2/GD2 synthase
  SIAT 2
  SIAT2
  UDP Gal:betaGlcNAc beta 1 4 N acetylgalactosaminyltransferase transferase 1
  UDP N acetyl alpha D galactosamine:(N acetylneuraminyl).

  more

• Anti-B4GALNT1/GM2 synthase antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (B4GALNT1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig2: Sample:
  Stomach (Mouse) Lysate at 40 ug
  Primary: Anti- B4GALNT1/GM2 synthase  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 59 kD
  Observed band size: 59 kD
  

  

  Fig3: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti- B4GALNT1/GM2 synthase  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 59 kD
  Observed band size: 53 kD

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