Fig3: Flow cytometric analysis of TRPML3 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-21, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).