Anti-BLOS1 antibody

Anti-BLOS1 antibody

• 种属反应性Rice,Arabidopsis thaliana

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原Synthetic peptide within thaliana BLOS1 aa 31-70.

• 偶联Non-conjugated

• Anti-BLOS1 antibody性能

• 形态Liquid

• 浓度1 mg/mL

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Endosome, cytoplasm.

• 其它名称Biogenesis of lysosome-related organelles complex 1 subunit 1 antibody
  BL1S1_HUMAN antibody
  BLOC-1 subunit 1 antibody
  Bloc1s1 antibody
  BLOS1 antibody
  GCN5-like protein 1 antibody
  GCN5L1 antibody
  Protein RT14 antibody
  RT14 antibody

  more

• Anti-BLOS1 antibody应用

  WB:1:500-1:2,000
  IHC-P:1:50-1:200

• 

  Fig1: Western blot analysis of BLOS1 on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-BLOS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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