Anti-Connexin 45 / GJA7 / Cx45 antibody

Anti-Connexin 45 / GJA7 / Cx45 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within Human Connexin 45 aa 280-410.

• 偶联Non-conjugated

• Anti-Connexin 45 / GJA7 / Cx45 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cell membrane, Gap junction.

• 其它名称Connexin 45 / GJA7 antibody
  Connexin 45 antibody
  Connexin-45 antibody
  Connexin45 antibody
  CX 31.9 antibody
  CX 45 antibody
  CX31.9 antibody
  Cx45 antibody
  CXG1_HUMAN antibody
  DKFZp686P0738 antibody
  Gap junction alpha 7 protein antibody
  Gap junction alpha-7 protein antibody
  Gap junction gamma 1 protein antibody
  Gap junction gamma-1 protein antibody
  Gap junction protein alpha 7 45kDa antibody
  Gap junction protein alpha 7 antibody
  Gap junction protein, gamma 1, 45kDa antibody
  GJA 7 antibody
  GJA7 antibody
  GJC1 antibody
  GJD3 antibody

  more

• Anti-Connexin 45 / GJA7 / Cx45 antibody应用

  WB:1:500-1:1000
  ICC:1:500-1:100
  IHC-P:1:50-1:100

• 

  Fig1: Western blot analysis of Connexin 45 / GJA7 / Cx45 on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  
  Lane 1: Anti-Connexin-45 Antibody;
  Lane 2: Anti-Connexin-45 Antibody, pre-incubated with the
  immunizaiton peptide.

  

  Fig2: ICC staining Connexin 45 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining Connexin 45 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues we

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