Anti-Cytokeratin 14 antibody

Anti-Cytokeratin 14 antibody

• 种属反应性Human,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原Synthetic peptide within C-terminal Human Cytokeratin 14.

• 偶联Non-conjugated

• Anti-Cytokeratin 14 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Cytoplasm. Nucleus.

• 其它名称CK 14 antibody
  CK-14 antibody
  ck14 antibody
  Cytokeratin 14 antibody
  Cytokeratin-14 antibody
  Cytokeratin14 antibody
  Dowling Meara antibody
  EBS3 antibody
  EBS4 antibody
  Epidermolysis bullosa simplex antibody
  K14 antibody
  K1C14_HUMAN antibody
  Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
  Keratin 14 antibody
  Keratin antibody
  Keratin type I cytoskeletal 14 antibody
  Keratin, type I cytoskeletal 14 antibody
  Keratin-14 antibody
  Keratin14 antibody
  Koebner antibody
  Krt 14 antibody
  Krt14 antibody
  NFJ antibody
  OTTHUMP00000164624 antibody
  type I cytoskeletal 14 antibody

  more

• Anti-Cytokeratin 14 antibody应用

  WB:1:1,000-1:5,000
  IHC-P:1:50-1:200

• 

  Fig1: Western blot analysis of Cytokeratin 14 on rat skin tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of Cytokeratin 14 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 14 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Cytokeratin 14 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

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