Anti-G-protein coupled receptor 30 antibody

Anti-G-protein coupled receptor 30 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用FC,WB,ICC,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within Human G-protein coupled receptor 30 aa 270-380.

• 偶联Non-conjugated

• Anti-G-protein coupled receptor 30 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Mitochondrion,Nucleus,Endosome,Golgi apparatus,Endoplasmic reticulum,Cytoskeleton.

  
  SwissProt: O08878 Rat
  

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• Anti-G-protein coupled receptor 30 antibody应用

  WB:1:500-1:1,000
  ICC:1:50-1:200
  IHC-P:1:50-1:200
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of G-protein coupled receptor 30 on Lovo cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining G-protein coupled receptor 30 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody  at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining G-protein coupled receptor 30 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining G-protein coupled receptor 30 in SH-SY-5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibodyat a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissue

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissue

  

  Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues

  

  Fig10: Flow cytometric analysis of G-protein coupled receptor 30 was done on SH-SY-5Y cells. The cells were fixed, permeabilized and stained with G-protein coupled receptor 30 antibody at 1/100 dilution (red) compared with an unlabelled control (cells wit

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