Anti-Cytokeratin 8 antibody
种属反应性Human
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Recombinant protein within Human Cytokeratin 8 aa 380-500.
偶联Non-conjugated
Anti-Cytokeratin 8 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Cytoplasm. Nucleus.
其它名称CARD2 antibody
CK 8 antibody
CK-8 antibody
CK8 antibody
CYK8 antibody
CYKER antibody
Cytokeratin endo A antibody
Cytokeratin-8 antibody
DreK8 antibody
EndoA antibody
K2C8 antibody
K2C8_HUMAN antibody
K8 antibody
Keratin 8 antibody
Keratin type II cytoskeletal 8 antibody
Keratin, type II cytoskeletal 8 antibody
Keratin-8 antibody
KO antibody
Krt 2.8 antibody
KRT8 antibody
MGC118110 antibody
MGC174782 antibody
MGC53564 antibody
MGC85764 antibody
sb:cb186 antibody
Type-II keratin Kb8 antibody
more
Anti-Cytokeratin 8 antibody应用
WB:1:500-1:2,000
ICC:1:50
IHC-P:1:50-1:200
FC:1:50-1:100
Fig1: Western blot analysis of Cytokeratin 8 on K562 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining Cytokeratin 8 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 8 at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cytokeratin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibodyat 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig6: Flow cytometric analysis of Cytokeratin 8 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Cytokeratin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-Rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
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