Anti-IL-22 antibody

Anti-IL-22 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用ELISA,WB,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within human IL-22 aa 34-179.

• 偶联Non-conjugated

• Anti-IL-22 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein A purified.

• 亚细胞定位Secreted.

• 其它名称Cytokine Zcyto18 antibody
  IL 10 related T cell derived inducible factor antibody
  IL 21 antibody
  IL 22 antibody
  IL D110 antibody
  IL TIF antibody
  IL-10-related T-cell-derived-inducible factor antibody
  IL-22 antibody
  IL-TIF antibody
  IL21 antibody
  Il22 antibody
  IL22_HUMAN antibody
  ILD110 antibody
  ILTIF antibody
  Interleukin 10 related T cell derived inducible factor antibody
  interleukin 21 antibody
  Interleukin 22 antibody
  Interleukin-22 antibody
  MGC79382 antibody
  MGC79384 antibody
  TIFa antibody
  TIFIL 23 antibody
  TIFIL23 antibody
  UNQ3099/PRO10096 antibody
  zcyto18 antibody

  more

• Anti-IL-22 antibody应用

  ELISA:1:5000-1:10,000
  WB:1:500
  IHC-P:1:50-1:200

• 

  Fig1: Western blot analysis of IL-22 on IL22 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human breasr cancer tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibodyat 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

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