Anti-Estrogen Receptor alpha antibody

Anti-Estrogen Receptor alpha antibody

• 种属反应性Human, Mouse, Rat

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原Recombinant protein within Human Estrogen Receptor alpha aa 1-120.

• 偶联Non-conjugated

• Anti-Estrogen Receptor alpha antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cytoplasm. Nucleus. Cell membrane. Golgi apparatus.

• 其它名称DKFZp686N23123 antibody
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• Anti-Estrogen Receptor alpha antibody应用

  WB: 1:500-1:1,000
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of Estrogen Receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: 293T cell lysate
  Lane 2: Rat ovary tissue lysate

  

  Fig2: Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

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