Anti-CD13 antibody

Anti-CD13 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Recombinant protein within Human CD13 aa 280-450.

• 偶联Non-conjugated

• Anti-CD13 antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cell membrane.

• 其它名称Alanyl (membrane) aminopeptidase antibody
  Alanyl aminopeptidase antibody
  Aminopeptidase M antibody
  Aminopeptidase N antibody
  AMPN_HUMAN antibody
  ANPEP antibody
  AP M antibody
  AP N antibody
  AP-M antibody
  AP-N antibody
  APN antibody
  CD 13 antibody
  CD13 antibody
  CD13 antigen antibody
  gp150 antibody
  hAPN antibody
  LAP 1 antibody
  LAP1 antibody
  Microsomal aminopeptidase antibody
  Myeloid plasma membrane glycoprotein CD13 antibody
  p150 antibody
  PEPN antibody

  more

• Anti-CD13 antibody应用

  WB:1:500
  ICC:1:100-1:400
  IHC-P:1:50-1:200
  FC:1:50-1:100

  Fig1: Western blot analysis of CD13 on rat kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining CD13 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CD13 polyclonal antibody at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining CD13 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CD13 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat kdiney tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibodyat 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human kdiney tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA fo

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for

  

  Fig9: Flow cytometric analysis of CD13 was done on A549 cells. The cells were fixed, permeabilized and stained with CD13 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After in

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