Anti-delta 1 Catenin/CAS antibody

Anti-delta 1 Catenin/CAS antibody

• 种属反应性Human,Rat

• 验证应用WB,ICC

• 抗体类型兔多抗

• 免疫原Recombinant protein within human delta 1 Catenin/CAS aa 130-320.

• 偶联Non-conjugated

• Anti-delta 1 Catenin/CAS antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein A purified.

• 亚细胞定位Cell membrane, Cytoplasm, Membrane, Nucleus. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.

• 数据链接SwissProt: O60716 Human
  

• 其它名称Cadherin associated Src substrate antibody
  Cadherin-associated Src substrate antibody
  CAS antibody
  Catenin (cadherin associated protein) delta 1 antibody
  Catenin delta 1 antibody
  Catenin delta antibody
  Catenin delta-1 antibody
  CTND1_HUMAN antibody
  CTNND 1 antibody
  CTNND antibody
  CTNND1 antibody
  delta 1 Catenin antibody
  KIAA0384 antibody
  p120 antibody
  P120 CAS antibody
  p120 catenin antibody
  P120 CTN antibody
  p120(cas) antibody
  p120(ctn) antibody
  P120CAS antibody
  P120CTN antibody

  more

• Anti-delta 1 Catenin/CAS antibody应用

  WB:1:500
  ICC:1:50-1:200

  Fig1: Western blot analysis of delta 1 Catenin/CAS on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining delta 1 Catenin/CAS in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining delta 1 Catenin/CAS in PMVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining delta 1 Catenin/CAS in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

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