Anti-Annexin A1 antibody

Anti-Annexin A1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within human Annexin A1 aa 150-310.

• 偶联Non-conjugated

• Anti-Annexin A1 antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Secreted.

• 其它名称Annexin 1 antibody
  Annexin A1 antibody
  Annexin I (lipocortin I) antibody
  Annexin I antibody
  Annexin-1 antibody
  AnnexinA1 antibody
  AnnexinI antibody
  ANX 1 antibody
  ANX A1 antibody
  ANX1 antibody
  ANXA 1 antibody
  ANXA1 antibody
  ANXA1 protein antibody
  ANXA1_HUMAN antibody
  Calpactin 2 antibody
  Calpactin II antibody
  Calpactin-2 antibody
  CalpactinII antibody
  Chromobindin 9 antibody
  Chromobindin-9 antibody
  Chromobindin9 antibody
  HGNC:533 antibody
  Lipocortin 1 antibody
  Lipocortin I antibody
  Lipocortin1 antibody
  LipocortinI antibody
  LPC 1 antibody
  LPC1 antibody
  p35 antibody
  Phospholipase A2 inhibitory protein antibody

  more

• Anti-Annexin A1 antibody应用

  WB: 1:500-1:2,000
  ICC:1:50-1:200
  IHC-P:1:200

• 

  Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: A431 cell lysate
  Lane 2: Rat uterus tissue lysate

  

  Fig2: ICC staining Annexin A1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining Annexin A1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining Annexin A1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in

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