Anti-SEL1L antibody

Anti-SEL1L antibody

• 种属反应性Zebrafish

• 验证应用WB,IF,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within zebrafish SEL1L aa 100-300.

• 偶联Non-conjugated

• Anti-SEL1L antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cytoplasm.

• 其它名称IBD2 antibody
  PRO1063 antibody
  Protein sel-1 homolog 1 antibody
  SE1L1_HUMAN antibody
  Sel 1 like protein antibody
  Sel 1 suppressor of lin 12 like antibody
  Sel-1L antibody
  SEL1 LIKE antibody
  Sel1l antibody
  SEL1LIKE antibody
  Suppressor of lin-12-like protein 1 antibody
  TSA305 antibody
  UNQ128 antibody
  UNQ128/PRO1063 antibody

  more

• Anti-SEL1L antibody应用

  WB: 1:500
  IF:1:50-1:200
  IHC-P:1:200

• 

  Fig1: Western blot analysis of SeL1L on Zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Immunofluorescence staining of paraffin-embedded zebrafish using anti-SEL1L rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SeL1L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

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