Anti-CDKN2A/p16INK4a antibody

Anti-CDKN2A/p16INK4a antibody

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within C-terminal human CDKN2A/p16INK4a.

• 偶联Non-conjugated

• Anti-CDKN2A/p16INK4a antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Nucleus. Cytoplasm.

• 其它名称CCM2 antibody
  CDK4 inhibitor p16 INK4 antibody
  CDK4I antibody
  CDKN2 antibody
  CDKN2A antibody
  Cell cycle negative regulator beta antibody
  CMM2 antibody
  Cyclin dependent kinase 4 inhibitor A antibody
  Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
  Cyclin Dependent Kinase Inhibitor 2A antibody
  Cyclin dependent kinase inhibitor 2A isoform 4 antibody
  Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
  Cyclin dependent kinase inhibitor p16 antibody
  INK4 antibody
  INK4A antibody
  MLM antibody
  MTS1 antibody
  Multiple tumor suppressor 1 antibody
  p14 antibody
  p16 antibody
  P16INK4 antibody
  p16INK4a antibody
  p19 antibody
  p19Arf antibody
  TP16 antibody

  more

• Anti-CDKN2A/p16INK4a antibody应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of CDKN2A/p16INK4a on SiHa cell lysate using anti-CDKN2A/p16INK4a antibody at 1/500 dilution.
  Positive control:
  Lane 1: SiHa cell lysate
  Lane 2: SiHa cell lysate with immunization peptide
  

  

  Fig2: ICC staining CDKN2A/p16INK4a in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining CDKN2A/p16INK4a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining CDKN2A/p16INK4a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDKN2A/p16INK4a antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-CDKN2A/p16INK4a antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  

  Fig7: Flow cytometric analysis of 293T cells with CDKN2A/p16INK4a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the

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