Anti-APR3 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Synthetic peptide corresponding to Mouse KIF3A C terminal.
偶联Non-conjugated
Anti-APR3 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Plasma membrane, Nucleus envelope.
其它名称APR-3
ATRAID
APR3
C2orf28
HSPC013
UNQ214/PRO240
Anti-APR3 antibody应用
WB: 1:500-2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.
Lane 1: Anti-APR3 antibody (1/500).
Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.
Fig2: ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-APR3 antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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