Anti-Sodium hydrogen exchanger 1 antibody

Anti-Sodium hydrogen exchanger 1 antibody

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human NHE1 470-550/815

• 偶联Non-conjugated

• Anti-Sodium hydrogen exchanger 1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A.

• 亚细胞定位Membrane; Multi-pass membrane protein.

  
  

• 其它名称amiloride-sensitive
  APNH
  APNH1
  FLJ42224
  Na Li countertransporter
  Na(+)/H(+) antiporter
  Na(+)/H(+) exchanger 1
  Na+ H+ antiporter amiloride-sensitive
  Na+ H+ antiporter
  Na+ H+ exchanger 1
  NHE-1
  NHE1
  OTTHUMP00000004468
  SL9A1_HUMAN
  SLC9A1
  Sodium hydrogen exchanger 1
  Sodium/hydrogen exchanger 1
  solute carrier family 9
  Solute carrier family 9 member 1
  Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive
  Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1
  Solute carrier family 9 subfamily A member 1.

  more

• Anti-Sodium hydrogen exchanger 1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Brain(Rat) lysate at 30ug;
  Liver(Rat) lysate at 30ug;
  Primary: Anti-NHE1  at 1:200 dilution;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
  Predicted band size : 91kD
  Observed band size : 91kD
  We are unsure as to the identity of these extra bands

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig3: Sample:
  molt-4(human)cell Lysate at 40 ug
  Primary: Anti- Sodium hydrogen exchanger 1  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 91kD
  Observed band size: 91 kD
  

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！