种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,Guinea Pig
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human SNF2H 901-1000/1052
偶联Non-conjugated
Anti-SNF2H antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus.
其它名称4933427E24Rik
ATP-dependent chromatin remodeling protein SNF2H
D030040M08Rik
D330027N15Rik
EC 3.6.1.-
hISWI
hSNF2H
ISWI
MommeD4
SMARCA5
SMCA5
SMCA5_HUMAN
Snf2h
Sucrose nonfermenting like 5
Sucrose nonfermenting protein 2 homolog
Sucrose nonfermenting, yeast, homolog of
sucrose nonfermenting-like 5
SWI/SNF related matrix associated actin dependent regulator of chromatin a5
SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 5
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
WCRF135.
more
Anti-SNF2H antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-SNF2H t 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 122 kD
Observed band size: 127 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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