Anti-MADH7/Smad7 antibody
种属反应性Human,Mouse,Rat,Pig,Cow
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Smad7 1-100/426
偶联Non-conjugated
Anti-MADH7/Smad7 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytopl
数据链接SwissProt: O15105 Human
其它名称hSMAD 7
hSMAD7
MAD (mothers against decapentaplegic Drosophila) homolog 7
MAD
Mad homolog 7
MAD mothers against decapentaplegic homolog 7
MADH 7
MADH 8
MADH8
Mothers Against Decapentaplegic Drosophila Homolog of 7
Mothers against decapentaplegic homolog 7
Mothers against decapentaplegic homolog 8
Mothers against DPP homolog 7
Mothers against DPP homolog 8
SMA- AND MAD-RELATED PROTEIN 7
SMAD 7
SMAD
SMAD family member 7
SMAD, mothers against DPP homolog 7 (Drosophila)
SMAD, mothers against DPP homolog 7
Smad7
SMAD7_HUMAN.
more
应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
Lung(Mouse) Lysate at 30 ug
Placenta(Mouse) Lysate at 30 ug
Large intestine(Mouse) Lysate at 30 ug
Primary: Anti- MADH7/Smad7 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 50 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Smad7) Polyclonal Antibody, Unconjugated at 1:600 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig3: Sample:Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- MADH7/Smad7 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 50 kD
Fig4: Sample:Spleen(Mouse) Lysate at 30 ug
Primary: Anti- MADH7/Smad7 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 50 kD
Fig5: Sample:Stomach (Mouse) Lysate at 30 ug
Primary: Anti- MADH7/Smad7 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 50 kD
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