Anti-Lubricin antibody

Anti-Lubricin antibody

• 种属反应性Human,Mouse,Rat,Dog,Cow,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Lubricin/SZP 1151-1250/1404

• 偶联Non-conjugated

• Anti-Lubricin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Secreted.

• 数据链接SwissProt: Q92954 Human
  

• 其它名称Superficial zone proteoglycan
  articular superficial zone protein
  bG174L6.2
  CACP
  camptodactyly arthropathy coxa vara pericarditis syndrome gene
  FLJ32635
  HAPO
  Jacobs camptodactyly-arthropathy-pericarditis syndrome gene
  JCAP
  megakaryocyte stimulating factor
  MSF
  PRG 4
  PRG4
  proteoglycan 4
  Proteoglycan4
  Superficial zone proteoglycan
  SZP
  PRG4_HUMAN.

  more

• Anti-Lubricin antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Lubricin) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig2: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti-Lubricin at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 152 kD
  Observed band size: 152 kD

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