Anti-Integrin alpha 3 antibody

Anti-Integrin alpha 3 antibody

• 种属反应性Human,Mouse,Rat,Pig

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Integrin alpha 3 light chain 976-1025/1051

• 偶联Non-conjugated

• Anti-Integrin alpha 3 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein.

• 数据链接SwissProt: P26006 Human
  

• 其它名称Integrin alpha 3 light chain
  AA407068
  Antigen identified by monoclonal
  Integrin alpha-3
  J143
  CD49 antigen like family member C
  CD49 antigen-like family member C
  CD49c
  CD49c antigen
  FLJ34631
  FLJ34704
  FRP 2
  FRP-2
  FRP2
  Galactoprotein B3
  GAP B3
  GAPB3
  Integrin alpha 3
  Integrin alpha-3 light chain
  Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
  ITA3_HUMAN
  ITGA 3
  Itga3
  MSK18
  VCA 2
  VCA2
  Very late activation protein 3 receptor alpha 3 subunit
  VL3A
  VLA 3 alpha chain
  VLA 3 subunit alpha
  VLA-3 subunit alpha
  VLA3a.

  more

• Anti-Integrin alpha 3 antibody应用

  WB:1:500-2000
  FC:1μg /test

• 

  Fig1: Blank control: U937(blue).
  Primary Antibody: Rabbit Anti-Integrin alpha 3 antibody(ER1911-51), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-51, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

  

  Fig2: Sample: Lung(Mouse) lysate at 30ug;
  Primary: Anti-Integrin alpha 3 (ER1911-51) at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
  Predicted band size: 20/114 kD
  Observed band size: 20 kD

  

  Fig3: Sample: Intestinal(Mouse) lysate at 30ug;
  Primary: Anti-Integrin alpha 3 (ER1911-51) at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
  Predicted band size: 20/114 kD
  Observed band size: 24 kD

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