Anti-IL17C antibody

Anti-IL17C antibody

• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human IL-17C 101-197/197

• 偶联Non-conjugated

• Anti-IL17C antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Secreted.

• 数据链接SwissProt: Q9P0M4 Human
  

• 其它名称Interleukin-17C
  IL17C
  CX2
  IL-17C
  IL 17C
  IL-21
  IL 21
  MGC126884
  MGC138401
  Cytokine CX2
  IL17C_HUMAN.

  more

• Anti-IL17C antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Lane 1: Lung(Rat) Lysate at 30 ug
  Lane 2: Pancreas(Rat) Lysate at 30 ug
  Primary: Anti-IL-17C (ER1911-25) at 1:200 dilution;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
  Predicted band size : 20kD
  Observed band size : 22kD
  

  

  Fig2: Tissue/cell: Human colon cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-IL17C Polyclonal Antibody, Unconjugated(ER1911-25) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL17C) Polyclonal Antibody, Unconjugated (ER1911-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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