Anti-ICOS ligand antibody

Anti-ICOS ligand antibody

• 种属反应性Human,Rat

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human ICOS ligand 19-120/302

• 偶联Non-conjugated

• Anti-ICOS ligand antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leuko

• 数据链接SwissProt: O75144 Human
  

• 其它名称B7 H2
  B7 homolog 2
  B7 homologue 2
  B7 like protein Gl50
  B7 related protein 1
  B7-like protein Gl50
  B7-related protein 1
  B7-H2
  B7H2
  B7RP 1
  B7RP-1
  B7RP1
  CD 275
  CD275
  CD275 antigen
  GL 50
  GL50
  ICOS L
  ICOS LG
  ICOS ligand
  ICOSL
  ICOSL_HUMAN
  Icoslg
  Inducible T cell co stimulator ligand
  KIAA0653
  LICOS
  Transmembrane protein B7 H2 ICOS ligand
  ICOSLG
  ICOS ligand-like
  ICOS ligand.

  more

• Anti-ICOS ligand antibody应用

  WB:1:500-2000
  FC:1μg/Test

• 

  Fig1: Blank controlBlue):Jurkat cells (fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti-ICOS ligand antibody(ER1911-03), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-03, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

  

  Fig2: Sample:
  Jurkat(Human) Cell Lysate at 30 ug
  Primary: Anti-ICOS ligand (ER1911-03) at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 34 kD
  Observed band size: 35 kD

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