Anti-ICAM5 antibody
种属反应性Human,Mouse,Rat,Pig,Cow
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human ICAM5 501-600/924
偶联Non-conjugated
Anti-ICAM5 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Membrane; Single-pass type I membrane protein.
数据链接SwissProt: Q9UMF0 Human
其它名称ICAM5_HUMAN
ICAM5 precursor
Intercellular adhesion molecule 5
Intercellular adhesion molecule 5 precursor
Intercellular adhesion molecule 5, telencephalin
Telencephalin
TLCN
TLN.
more
Anti-ICAM5 antibody应用
WB:1:500-2000
FC:0.2ug/test
Fig1: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- ICAM5 (ER1911-02) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 167 kD
Fig2: Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-ICAM5 antibody (ER1911-02)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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