Anti-Alpha-tubulin antibody
种属反应性Human, Mouse, Rat, Zebrafish
验证应用WB, IHC-P, ICC, FC, IF
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
Anti-Alpha-tubulin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm ,cytoskeleton
数据链接P68366
其它名称TUBA1 antibody
TUBA1A antibody
TUBA2 antibody
TUBA3 antibody
TUBA3C antibody
TUBA4A antibody
Tubulin, alpha 1a antibody
Tubulin, alpha 3c antibody
Tubulin, alpha 4a antibody
more
Anti-Alpha-tubulin antibody应用
WB: 1:5,000-1:10,000
ICC: 1:200-1:500
IHC-P: 1:200-1:500
IF: 1:200-1:500
FC: 1:50-1:100
Fig1: Western blot analysis of Alpha-tubulin on different cell lysates using anti-Alpha -tubulin antibody at 1/10000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: HepG2
Lane 3: PC12
Lane 4: Hela
Fig2: ICC staining of Alpha-tubulin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining of Alpha-tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: IHC-P image of Alpha-tubulin staining with Alpha-tubulin antibody on tissue sections from human stomach. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with Alpha-tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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