Anti-Alpha smooth muscle Actin antibody
种属反应性Human,Mouse,Rat
验证应用WB, ICC, IHC-P
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
Anti-Alpha smooth muscle Actin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoskeleton
数据链接P62736 Human
P62737 Mouse
P62738 Rat
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Anti-Alpha smooth muscle Actin antibody应用
WB: 1:2,000
ICC: 1:200
IHC-P: 1:200
Fig1: Western blot analysis of alpha smooth muscle actin on different cell lysates using anti-Alpha smooth muscle actin antibody at 1/2000 dilution.
Positive control:
Lane 1: Jurkat
Lane2 : NIH/3T3
Lane 3: Mouse embryonic stem cell
Lane 4: Mouse colon
Fig2: ICC staining of Alpha smooth muscle actin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining of Alpha smooth muscle actin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Alpha smooth muscle actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-Alpha smooth muscle actin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Alpha smooth muscle actin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Alpha smooth muscle actin antibody. Counter stained with hematoxylin.
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