Anti-N-Cadherin antibody

Anti-N-Cadherin antibody

• 种属反应性Human, Mouse

• 验证应用WB, ICC, IHC-P, FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide (KLH-coupled) within human N-cadherin 360-420 aa.

• 偶联Non-conjugated

• Anti-N-Cadherin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cell membrane

• 数据链接SwissProt: P19022 Human
  SwissProt: P15116 Mouse
  

• 其它名称CADH2_HUMAN antibody
  Cadherin 2 antibody
  Cadherin 2 N cadherin neuronal antibody
  Cadherin 2 type 1 antibody
  Cadherin 2 type 1 N cadherin neuronal antibody
  Cadherin 2, type 1, N-cadherin (neuronal) antibody
  Cadherin-2 antibody
  Cadherin2 antibody
  Calcium dependent adhesion protein neuronal antibody
  CD325 antibody
  CD325 antigen antibody
  CDH2 antibody
  CDHN antibody
  CDw325 antibody
  CDw325 antigen antibody
  N cadherin 1 antibody
  N-cadherin antibody
  NCAD antibody
  Neural cadherin antibody
  OTTHUMP00000066304 antibody
  OTTHUMP00000067378 antibody

  more

• Anti-N-Cadherin antibody应用

  WB: 1:500
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:50

• 

  Fig1: Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.
  Positive control:
  Lane 1: Mouse heart lysates
  Lane2 : Human heart lysates

  

  Fig2: ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.

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