Anti-Osteopontin antibody
种属反应性Human, Zebrafish
验证应用WB, IHC-P
抗体类型兔多抗
免疫原Synthetic peptide within human aa 280-310.
偶联Non-conjugated
Anti-Osteopontin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Immunogen affinity purified
亚细胞定位Cytosol
数据链接SwissProt: P10451 Human
其它名称BNSP antibody
Bone sialoprotein 1 antibody
BSP I antibody
BSPI antibody
Early T lymphocyte activation 1 antibody
ETA 1 antibody
ETA1 antibody
MGC110940 antibody
Nephropontin antibody
OPN antibody
Osteopontin antibody
osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
OSTP_HUMAN antibody
PSEC0156 antibody
secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
Secreted phosphoprotein 1 antibody
SPP 1 antibody
SPP-1 antibody
SPP1 antibody
SPP1/CALPHA1 fusion antibody
Urinary stone protein antibody
Uropontin antibody
more
Anti-Osteopontin antibody应用
WB: 1:1,000
Fig1: Western blot analysis on human thymus using anti-Osteopontin polyclonal antibody.
Fig2: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-8, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-8, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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