Anti-Osteopontin antibody

Anti-Osteopontin antibody

• 种属反应性Human, Zebrafish

• 验证应用WB, IHC-P

• 抗体类型兔多抗

• 免疫原Synthetic peptide within human aa 280-310.

• 偶联Non-conjugated

• Anti-Osteopontin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Immunogen affinity purified

• 亚细胞定位Cytosol

• 数据链接SwissProt: P10451 Human
  

• 其它名称BNSP antibody
  Bone sialoprotein 1 antibody
  BSP I antibody
  BSPI antibody
  Early T lymphocyte activation 1 antibody
  ETA 1 antibody
  ETA1 antibody
  MGC110940 antibody
  Nephropontin antibody
  OPN antibody
  Osteopontin antibody
  osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
  OSTP_HUMAN antibody
  PSEC0156 antibody
  secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
  Secreted phosphoprotein 1 antibody
  SPP 1 antibody
  SPP-1 antibody
  SPP1 antibody
  SPP1/CALPHA1 fusion antibody
  Urinary stone protein antibody
  Uropontin antibody

  more

• Anti-Osteopontin antibody应用

  WB: 1:1,000

• 

  Fig1: Western blot analysis on human thymus using anti-Osteopontin polyclonal antibody.

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-8, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-8, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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