血小板内皮细胞黏附分子1抗体

血小板内皮细胞黏附分子1抗体

英文名称    CD31    

中文名称    血小板内皮细胞黏附分子1抗体    

别    名    platelet endothelial cell adhesion molecule precursor-1; PECAM-1; PECAM1; Adhesion molecule; CD31 antigen; CD31 EndoCAM; Endocam; FLJ34100; FLJ58394; GPIIA; Pecam 1; PECA1_HUMAN; PECAM 1 CD31 EndoCAM; PECA1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; Adhesion molecule; Platelet/endothelial cell adhesion molecule.      

研究领域    肿瘤  心血管  细胞生物  免疫学  干细胞  细胞粘附分子  血管内皮细胞  内皮细胞      

抗体来源    Mouse    

克隆类型    Monoclonal    

克 隆 号    3B5    

交叉反应    Human,     

产品应用    WB=1:100-1000  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    78kDa    

细胞定位    细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from human CD31: <Extracellular>    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Type I membrane protein. SIZE: 738 amino acids; 82536 Da. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. Tyr-690 plays a critical role in leukocyte transendothelial migration (TEM) and is required for efficient trafficking of CD31 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. CD31 prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of CD31 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of CD31 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).     

血小板内皮细胞黏附分子-1 ( Platelet/ endothelial cell adhesion molecule, PECAM-1)在血小板、内皮细胞、单核细胞、嗜中性细胞及某些T细胞亚群上表达的质膜糖蛋白，属于免疫球蛋白超基因家族成员，在细胞外结构域中有6个C2亚类免疫球蛋白样保守性同原单位。在炎症应答中起作用。