磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

英文名称    phospho-RPS6KA1 (Ser352)    

中文名称    磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体    

别    名    p90RSK (Phospho-Ser352); RPS6KA1(phospho S352); p90 RSK1 ; p90rsk ; p90S6K ; pp90RSK1 ; Ribosomal protein S6 kinase 90kD 1 ; Ribosomal protein S6 kinase 90kD polypeptide 1 ; Ribosomal protein S6 kinase 90kDa polypeptide 1 ; Ribosomal protein S6 kinase alpha 1 ; Ribosomal protein S6 kinase polypeptide 1 ; Ribosomal S6 kinase 1 ; RPS6K1 alpha ; rps6ka ; Rps6ka1 ; RSK 1 ; RSK 1 p90 ; RSK ; RSK1 ; S6K alpha 1 ; 90 kDa ribosomal protein S6 kinase 1 ; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 ; dJ590P13.1 ; EC 2.7.11.1 ; HU 1 ; HU1 ; MAP kinase activated protein kinase 1a ; MAPKAP kinase 1A ; MAPKAPK1A ; MGC79981 ; Mitogen-activated protein kinase-activated protein kinase 1A; KS6A1_MOUSE.      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  信号转导  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Mouse, Rat,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    81kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from mouse RPS6KA1 around the phosphorylation site of Ser352:RD(p-S)PG     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.    

p90RSK蛋白属于广泛表达的丝氨酸/苏氨酸激酶。RSK家族包括RSK1、RSK2、RSK3和RSK4，它们的典型特征是含有两个不同的功能性激酶结构域和羧基端ERKs(extracellular signal-regulated kinases)结合结构域。RSK激酶结构域内外的几个位点包括Ser380、Thr359、Ser363和Thr573的磷酸化都对RSK的激酶活性激活非常重要。
RSK1-3可被MAPKs所磷酸化激活，也可以通过自身磷酸化激活，还可被一些生长因子或神经递质等诱导激活的PI3K信号通路所激活。RSK在被MAPK磷酸化激活时，是由Ras、Raf-1、MAP kinase kinase(MEK)和MAP kinase信号通路所介导。PI3K诱导的RSK1激活是由丝氨酸/苏氨酸激酶mTOR介导的。
RSK1在葡萄糖稳态调控和细胞大小的调控中起重要作用。RSK可以磷酸化并调节转录因子c-fos和糖原合成酶激酶3(GSK3,glycogen synthase kinase 3)的活性。有报道RSK1可以和MAPK1、IκBα、TOB和TSC2等相互作用。RSK可以在有丝分裂信号刺激下会转位到细胞核内。