T细胞调控相关蛋白抗体

T细胞调控相关蛋白抗体

英文名称    CRTAM    

中文名称    T细胞调控相关蛋白抗体    

别    名    CD355; Class I MHC restricted T cell associated molecule; Class-I MHC-restricted T-cell-associated molecule; CRTAM; CRTAM_HUMAN; Cytotoxic and regulatory T cell molecule; Cytotoxic and regulatory T-cell molecule.      

研究领域    细胞生物  细胞类型标志物  自然杀伤细胞  淋巴细胞  t-淋巴细胞      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Pig, Rabbit,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    42kDa    

细胞定位    细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from human CRTAM:31-130/393 <Extracellular>    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    CRTAM, (class-I MHC-restricted T-cell associated molecule), is an immunoglobulin-superfamily transmembrane protein and a new member of the nectin-like (Necls) family. In the immune system, CRTAM expression has been shown to be restricted to activated class-I MHC-restricted T cells, including NKT and CD8 T cells. CRTAM interacts with CADM1 and promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro, as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. Nectin-like (Necl) molecule 2, has been identified as a ligand of CRTAM. Necl2/CRTAM molecular pair could regulate a large panel of cell/cell interactions both within and outside of the immune system.    

CRTAM蛋白与调节细胞极性有关。细胞极性（cell polarity）的调控对于控制细胞的形态发生非常关键，并且这一过程在很多生物学功能中发挥着重要的作用，包括细胞分裂、分化、迁移等基础过程以及器官发生中的复杂行为等。细胞蛋白的空间结构排列是细胞极性形成的基础。抗原呈递细胞（antigen-presenting cell，APC）诱导T细胞的激活，而这会导致免疫突触（immunological synapse，IS）的形成、T细胞受体（TCR）接触信号衔接平台的组装、细胞支架的重组以及第二信使的产生等。