磷酸化胰岛素受体底物-1抗体

磷酸化胰岛素受体底物-1抗体

英文名称    phospho-IRS1 (Tyr612)    

中文名称    磷酸化胰岛素受体底物-1抗体    

别    名    p-IRS1; IRS1 (phospho-Tyr612); IRS1(phospho Y612); HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN.      

产品类型    磷酸化抗体     

研究领域    肿瘤  免疫学  信号转导  转录调节因子  糖尿病      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /Test IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    132kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Tyr612:DG(p-Y)MP     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.    

胰岛素受体底物-1是细胞中分子量为165-185kDa的磷蛋白，胰岛素受体的内源性底物。经胰岛素刺激后其酪氨酸残基被磷酸化。