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INTRODUCTION Following successful purification of small molecule drug candidates, it is necessary to pool the fractions, dry down those pooled fractions, and then prepare samples for analysis from this dried solid. Usually, the analysis of choice is a combination of analytical LCMS and NMR. To do this, it is necessary to re-dissolve the dried solid in a known quantity of a suitable solvent and then transfer small aliquots from this sample into the appropriate analysis tubes and vials.
INTRODUCTION A general liquid handling application is to use a liquid handler as an autosampler for a spectrophotometer. The traditional manual method for filling a flow-through cuvette is to use a peristaltic pump (a sipper) to draw the sample through the cuvette. This can easily be replicated using a Gilson GX liquid handler.
INTRODUCTION Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. The VERITY® 271 LCMS system (Figure 1) leverages the separating power of HPLC with the detection capabilities of MS to isolate fractions with high purity and achieve excellent recovery for target molecules. The system incorporates signal data from both ultraviolet (UV) and mass-based detectors.
INTRODUCTION Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. The VERITY® 271 LCMS system (Figure 1) leverages the separating power of HPLC with the detection capabilities of MS to isolate fractions with high purity and achieve excellent recovery for target molecules. The system incorporates signal data from both ultraviolet (UV) and mass-based detectors.