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MAbPac Protein A Affinty Column
MAbPac Protein A Column for Monoclonal Antibody Titer Analysis
The Thermo Scientific™ MAbPac™ Protein A column is a high-performance, affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody (MAb) concentration in the harvest cell culture (HCC). Early in the development of recombinant MAbs, a large of number of HCC samples must be screened for IgG titer. Affinity chromatography employing a Protein A ligand is often used to determine the MAb concentration. The MAbPac Protein A column is a high-pressure liquid chromatography (HPLC) column designed to provide fast, accurate, titer analysis with linearity over a wide concentration range without carryover.
The MAbPac Protein A column is based on a novel nonporous polymeric resin consisting of a hydrophobic divinylbenzene core and a hydrophilic surface. This proprietary resin is designed for fast mass transfer, resulting in efficient separations without carryover. The recombinant Protein A ligands are covalently attached onto the hydrophilic resin surface through their amine groups to provide a very rugged, reproducible column. The column is available in a HPLC bioinert, 4 × 35 mm PEEK column body.
Product Highlights:
? A novel nonporous polymeric resin with a hydrophilic surface
? Functionalized with the recombinant protein A ligands
? Wide range of MAb quantitation: 0.025–5 mg/mL
? Fast analysis: 2 min/analysis
? Rugged
? Low pressure: column pressure is less than 250 psi at 2 mL/min
The MAbPac Protein A column can be used for both titer analysis or concentrating and purifying IgG material for later experiments or two-dimensional (2D) chromatography.
| MAbPac Protein A Column Specifications | |
|---|---|
| Column Dimension | 4 × 35 mm |
| Base Matrix Material | Hydrophilic nonporous resin |
| Surface Ligand | Protein A |
| Particle Size | 12 μm |
| Binding capacity | ~3.5 mg IgG/g resin |
| Dynamic Loading Capacity | ≤100 μg IgG/column at 2 mL/min flow rate |
| pH Range | 1.9–7.5 |
| Maximum Flow rate | Up to 2 mL/min |
| Maximum Pressure | ≤1000 psi |
| Operating Temperature | <30 ?C |
| Analytical Columns | |
|---|---|
| MAbPac Protein A, 12 μm, Analytical Column (4 x 35mm) | 082539 |
企业名称
上海希言科学仪器有限公司
企业信息已认证
企业类型
有限责任公司(自然人投资或控股)
信用代码
91310117301434080L
成立日期
2014-05-26
注册资本
人民币100.0000万元整
经营范围
一般项目:技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;仪器仪表销售;实验分析仪器销售;光学仪器销售;货物进出口;技术进出口。(除依法须经批准的项目外，凭营业执照依法自主开展经营活动)
上海希言科学仪器有限公司
公司地址
上海市松江区九亭镇涞寅路1025号立同国际商务大厦3067室
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