PepSwift monolithic columns are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS. PepSwift columns are based on a polystyrene divinylbenzene copolymer bed. The bed structure of the column offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages for high-sensitivity proteomics applications.
Polymeric monolithic stationary phases
High-speed protein/peptide separations (< 10 min)
High separation efficiency (> 200.000 plates/m)
High column-to-column reproducibility
High sensitivity in LC/MS
Superior lifetime
Available in 100 μm, 200 μm and 500 μm i.d
Available in 5 and 25 cm length (only 100 and 200 μm i.d.)
nanoViper fitting
PepSwift Trap columns can be used for preconcentration and desalting of samples consisting of peptides and proteins without negative impact on the chromatographic performance or recovery of the compounds. Various ion-pairing agents can be used in the loading solvent and/or mobile phases to change the selectivity of the separation or improve the trapping efficiency.
PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Specifications | |||
---|---|---|---|
Typical Applications | Peptides, proteins, and oligonucleotides | ||
Column Dimensions | 100 μm × 50 mm | 200 μm × 50 mm | 500 μm × 50 mm |
Base Material | Polystyrene/divinyl benzene | Polystyrene/divinyl benzene | Polystyrene/divinyl benzene |
Chemistry | Phenyl | Phenyl | Phenyl |
Recommended Flow Rate | 700–1000 nL/min | 2–3 μL/min | 15–25 μL/min |
Temperature | 80 ?C | 80 ?C | 80 ?C |
Maximum Pressure | 4350 psi (30 MPa) | 4350 psi (30 MPa) | 4350 psi (30 MPa) |
Recommended Sample Quantity | 5 ng* | 20 ng* | 0.12 μg* |
Sample Capacity*** | 0.2 ng | 0.6 ng | 3.8 ng |
pH Stability | 1–10 | 1–10 | 1–10 |
Solvent Compatibility | Most common RP solvents | Most common RP solvents | Most common RP solvents |
*Tryptic digest of six proteins
**E. coli proteins
*** Dynamic loading capacity is defined as the 10% increase in peak width measured at half height induced by injected mass of a single peptide (tryptic peptide of cytochrome c for PepSwift RP) and intact protein (cytochrome c for ProSwift RP-10R), respectively
PepSwift and ProSwift Monolith Columns | |
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PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper | 164584 |
PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper | 164584 |
PepSwift Monolithic Nano Column, 100 μm i.d. x 25 cm, nanoViper | 164543 |
PepSwift Monolithic Capillary Column, 200 μm i.d. × 5 cm | 161409 |
PepSwift Monolithic Capillary Column, 200 μm i.d. x 5 cm, nanoViper | 164557 |
PepSwift Monolithic Capillary Column, 200 μm i.d. x 25 cm, nanoViper | 164542 |
PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm | 164087 |
PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm, nanoViper | 164585 |
ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper | 164586 |
ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper | 164586 |
PepSwift Trap Columns | |
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PepSwift Monolithic Trap Column, 200 μm x 5 mm, (set of 2), nanoViper | 164558 |
PepSwift Monolithic Trap Column, 200 μm x 5 mm, (set of 2), nanoViper | 164558 |
企业名称
上海希言科学仪器有限公司
企业信息已认证
企业类型
有限责任公司(自然人投资或控股)
信用代码
91310117301434080L
成立日期
2014-05-26
注册资本
人民币100.0000万元整
经营范围
一般项目:技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;仪器仪表销售;实验分析仪器销售;光学仪器销售;货物进出口;技术进出口。(除依法须经批准的项目外,凭营业执照依法自主开展经营活动)
上海希言科学仪器有限公司
公司地址
上海市松江区九亭镇涞寅路1025号立同国际商务大厦3067室
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