关注
已关注
已认证
粉丝量 0
400-860-5168转3944
仪器信息网认证电话，请放心拨打
ProPac IMAC Protein and Peptide Column
Analytical Column for Immobilized Metal Affinity Chromatography
The ProPac® IMAC-10 is an analytical HPLC column packed with 10 μm, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.
State-of-the-art technology for reusable columns with metal tailored specificity
Resolve target proteins using a single column in a high resolution gradient run
Retention control by imidazole concentration or pH gradient
High purity separations of metal-binding proteins
Wide range of metal-specific applications
The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions
The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).
| ProPac IMAC Columns Specifications | |
|---|---|
| Maximum Operating Pressure | <3000 psi (21 MPa) |
| Temperature Range | <60oC Recommended Temperature: Controlled ambient |
| Typical Operating Conditions | <600 psi at 1.0 mL/min (4 × 250mm) Recommended flow rate: 1.0 mL/min |
| Mobile Phase Compatibility | pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl |
| Resin Compostion | 10 μm diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts. |
| Metal Ion Capacity (μmol Cu) | >80 mmol Cu (4 × 250 mm analytical column) |
| Chelating Metals | Ni, Fe, Cu, others |
| Analytical Column Dimensions | 1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm 2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm |
| Loading Column Dimensions | 4 × 50 mm, 9 × 50 mm, 9 × 250 mm |
| Analytical Columns | |
|---|---|
| ProPac IMAC-10 Column (1 x 50 mm) | 063617 |
| ProPac IMAC-10 Column (2 x 50 mm) | 063272 |
| ProPac IMAC-10 Column (4 x 50 mm) | 063276 |
| ProPac IMAC-10 Column (9 x 50 mm) | 063615 |
| ProPac IMAC-10 Column (2 x 250 mm) | 063274 |
| ProPac IMAC-10 Column (4 x 250 mm) | 063278 |
| ProPac IMAC-10 Column (9 x 250 mm) | 063280 |
| ProPac IMAC-10 Column (22 x 250 mm) | 063282 |
| Accessories | |
|---|---|
| IMAC Loading Column (4 x 50 mm) | 063667 |
| IMAC Loading Column (9 x 50 mm) | 063710 |
| IMAC Loading Column (9 x 250 mm) | 063718 |
企业名称
上海希言科学仪器有限公司
企业信息已认证
企业类型
有限责任公司(自然人投资或控股)
信用代码
91310117301434080L
成立日期
2014-05-26
注册资本
人民币100.0000万元整
经营范围
一般项目:技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;仪器仪表销售;实验分析仪器销售;光学仪器销售;货物进出口;技术进出口。(除依法须经批准的项目外，凭营业执照依法自主开展经营活动)
上海希言科学仪器有限公司
公司地址
上海市松江区九亭镇涞寅路1025号立同国际商务大厦3067室
客服电话
