Anti-PRDM14抗体

Anti-PRDM14抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

505543Anti-PRDM14 Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,IF,IP,ChIP64kDa

Sensitivity  

Anti-PRDM14抗体Immunogen  Recombinant protein of human PRDM14

Host  Rabbit

Clone Number  

Antibody type  Polyclonal antibody

Anti-PRDM14抗体Purified method  Affinity purification

Isotype  Rabbit IgG

Formulation  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Anti-PRDM14抗体Dilution

WB~~1:500-1:2000

ICC~~1:50-1:200

IP~~1:20-1:100

IHC~~1:50-1:200

Other~~ChIP:1:20-1:100

Western blot analysis of extracts of various cell lines, using PRDM14 antibody.

Western blot analysis of various groups from PA-1 cell line, using PRDM14 antibody. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRDM14 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded human kidney using PRDM14 antibody at dilution of 1:100 (x400 lens).

Immunofluorescence analysis of A549 cell using PRDM14 antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cell using PRDM14 antibody.

Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.