Anti-B-Raf 抗体

Anti-B-Raf 抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

618122Anti-B-Raf Mouse mAbMouse mAbHuman,Mouse,Rat.WB, IF86kDa

Sensitivity  

Anti-B-Raf 抗体Immunogen  This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.

Host  Mouse

Clone Number  1F1-F7-A8

Anti-B-Raf 抗体Antibody type  Monoclonal antibody

Purified method  Affinity purified

Isotype  IgG1κ

Formulation  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-B-Raf 抗体Dilution

WB~~1:1000

ICC~~1:10~50

Western blot detection of B-Raf in A549,MCF7,3T3,C6 and Hela cell lysates using B-Raf mouse mAb (1:5000 diluted).Predicted band size:84KDa.Observed band size:86KDa.

Fluorescent image of C2C12 cell stained with BRAF Antibody(Cat#618122/SG100521B).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37℃. For secondary anti

Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35μg/lane). BRAF (arrow) was detected using the purified Mab.