Anti-EGF Receptor抗体

Anti-EGF Receptor抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

600419Anti-EGF Receptor Mouse mAbMouse mAbHuman,Mouse.WB, IHC-P,IF175kDa

Sensitivity  

Anti-EGF Receptor抗体Immunogen  This EGFR antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1163-1191 amino acids from the C-terminal region of human EGFR.

Host  Mouse

Clone Number  4D8-H11-G1

Antibody type  Monoclonal antibody

Anti-EGF Receptor抗体Purified method  Affinity purified

Isotype  IgG1

Formulation  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Dilution

WB~~1:1000

Anti-EGF Receptor抗体IHC~~1:25

Western blot detection of EGF Receptor in A549,MCF7,3T3 and Hela cell lysates using EGF Receptor mouse mAb (1:3000 diluted).Predicted band size:175KDa.Observed band size:175KDa.

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-EGF Receptor mouse mAb (dilution 1:200).

Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term)(Cat#600419). 600419 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, fol

Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term)(Cat#600419). 600419 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followe

Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term)(Cat#600419). 600419 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, follo

Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term)(Cat#600419). 600419 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, follo