Anti-UCHL1抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
600261Anti-UCHL1 Mouse mAbMouse mAbMouse,Rat.WB, IF, IHC-P27kDa
Sensitivity
Anti-UCHL1抗体Immunogen This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein.
Host Mouse
Clone Number 6E10-H4-G12
Anti-UCHL1抗体Antibody type Monoclonal antibody
Purified method Affinity purified
Isotype IgG1,κ
Formulation Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Anti-UCHL1抗体Dilution
WB~~1:1000
ICC~~1:25
IHC~~1:25
Application Images-expand |
![](http://www.zen-bioscience.com/uploads/AM1959B_wb_160921.jpg)
Western blot detection of UCHL1 in 3T3 and C6 cell lysates using UCHL1 mouse mAb (1:10000 diluted).Predicted band size:25KDa.Observed band size:27KDa.
![](http://www.zen-bioscience.com/uploads/AM1959b_IF_1.jpg)
Fluorescent image of A549 cells stained with UCHL1 Antibody(Cat#AM1959b). AM1959b was diluted at 1:25 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counte
![](http://www.zen-bioscience.com/uploads/AM1959b_IHC_1.jpg)
Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1(Cat#AM1959b). AM1959b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
![](http://www.zen-bioscience.com/uploads/AM1959b_WB_1.jpg)
UCHL1 Antibody(Cat. #AM1959b) western blot analysis in NCI-H460 cell line lysates (35μg/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).
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