Anti-β-Tubulin抗体

Anti-β-Tubulin抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

600116Anti-β-Tubulin Mouse mAbMouse mAbHuman,Mouse,Rat.IHC-P, WB, IF55kDa

Sensitivity  

Anti-β-Tubulin抗体Immunogen  TBB5 recombinant protein is used to produce this monoclonal antibody.

Host  Mouse

Clone Number  2H5-F6-A9

Antibody type  Monoclonal antibody

Anti-β-Tubulin抗体Purified method  Affinity purified

Isotype  IgG2b,κ

Formulation  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Dilution

WB~~1:1000

ICC~~1:25

Anti-β-Tubulin抗体IHC~~1:25

Western blot detection of β-Tubulin in A549,MCF7,3T3,C6 and Hela cell lysates using β-Tubulin mouse mAb (1:10000 diluted).Predicted band size:55KDa.Observed band size:55KDa.

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-β-Tubulin mouse mAb (dilution 1:200).

Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody(Cat#AM1031a). AM1031a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB s

Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance