Anti-Erk1抗体

Anti-Erk1抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

600460Anti-Erk1/2 Mouse mAbMouse mAbHuman,MouseFC, WB

Sensitivity  

Immunogen  Purified His-tagged Erk1/2 protein was used to produced this monoclonal Anti-Erk1抗体antibody.

Host  Mouse

Clone Number  784CT7.6.3

Antibody type  Monoclonal antibody

Anti-Erk1抗体Purified method  Affinity purified

Isotype  IgG2a

Formulation  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Dilution

WB~~1:4000

Anti-Erk1抗体FCM~~1:25

Western blot analysis of extracts from PC12,COS7,C6,3T3 and Hela cell lysates using Erk1/2 mouse mAb (1:500 diluted).Predicted band size:42,44KDa.Observed band size:42,44KDa.

Overlay histogram showing Jurkat cells stained with 600460(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-spe

Western blot analysis of extracts from HCC cell line and 293T cells, using mouse monoclonal antibody Erk1/2 Antibody (Cat. #600460).

Erk1/2 Antibody (Cat. #600460) western blot analysis in 293,MCF-7,Jurkat,mouse NIH/3T3,rat C6 cell line and mouse heart lysates (35μg/lane).This demonstrates the Erk1/2 antibody detected the Erk1/2 protein (arrow).