Anti-Akt抗体

Anti-Akt抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310081Anti-Akt(Phospho-Thr450) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC60

Sensitivity  The antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450.

Anti-Akt抗体Immunogen  Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.

Host  Rabbit

Clone Number  

Antibody type  Polyclonal antibody

Anti-Akt抗体Purified method  

Isotype  IgG

Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Dilution

WB~~1:500-1:1000

ICC~~1:100-1:200

Anti-Akt抗体IHC~~1:50-1:100

Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using AKT (phospho-Thr450) Rabbit pAb (310081,1:500 diluted,upper) and AKT(pan) Mouse mAb (200323,lower).

Immunofluorescence staining of methanol-fixed Hela cells using AKT1 (phospho-Thr450) Antibody .

Western blot analysis of extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450) Antibody .

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450) Antibody (left) or the same antibody preincubated with blocking peptide (right).