Anti-eIF2α抗体

Anti-eIF2α抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310073Anti-eIF2α(Phospho-Ser51) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC38

Sensitivity  The antibody detects endogenous level of eIF2α only when phosphorylated at serine 51.

Anti-eIF2α抗体Immunogen  Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2α.

Host  Rabbit

Clone Number  

Anti-eIF2α抗体Antibody type  Polyclonal antibody

Purified method  

Isotype  IgG

Anti-eIF2α抗体Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Dilution

WB~~1:500-1:1000

ICC~~1:100-1:200

IHC~~1:50-1:100

Western blot analysis of extracts from Hela cells, untreated or treated with UV, using eIF2α (Phospho-Ser51) Rabbit pAb (310073,1:500 diluted,upper) or eIF2α Mouse mAb (201137,lower).

Immunofluorescence staining of methanol-fixed Hela cells using eIF2α(Phospho-Ser51) Antibody .

Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2α (Phospho-Ser51) Antibody .

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51) Antibody (left) or the same antibody preincubated with blocking peptide #51279 (right).