Anti-EGFR抗体

Anti-EGFR抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310059Anti-EGFR(Phospho-Tyr845) Rabbit pAbRabbit pAbHuman,Mouse,RatWB175

Sensitivity  The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 869.

Anti-EGFR抗体Immunogen  Peptide sequence around phosphorylation site of tyrosine 869 (K-E-Y(p)-H-A) derived from Human EGFR.

Host  Rabbit

Clone Number  

Antibody type  Polyclonal antibody

Anti-EGFR抗体Purified method  

Isotype  IgG

Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Dilution

Anti-EGFR抗体WB~~1:500-1:1000

Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using EGFR (Phospho-Tyr845) Rabbit pAb (310059,1:500 diluted,upper) and EGF Receptor Mouse mAb (201012-3F12,lower).

Western blot analysis of extracts from MDA cells untreated or treated with EGF using EGFR (Phospho-Tyr869) Antibody .