Anti-NFκB-p65抗体

Anti-NFκB-p65抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310052Anti-NFκB-p65(Phospho-Ser529) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC65

Sensitivity  The antibody detects endogenous level of NFκB-p65 only when phosphorylated at serine 529.

Anti-NFκB-p65抗体Immunogen  Peptide sequence around phosphorylation site of serine 529 (L-L-S(p)-G-D) derived from Human NFκB-p65.

Host  Rabbit

Clone Number  

Anti-NFκB-p65抗体Antibody type  Polyclonal antibody

Purified method  

Isotype  IgG

Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Dilution

WB~~1:500-1:1000

ICC~~1:100-1:200

Anti-NFκB-p65抗体IHC~~1:50-1:100

Western blot analysis of extracts from 293 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Ser529) Antibody .

Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser529) Antibody .

Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody .

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser529) Antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot detection of NFkB-p65(Phospho-Ser529) in 3T3 and Hela cells untreated or treated with Alkaline phosphatase using NFkB-p65(Phospho-Ser529) Rabbit pAb (dilution 1:500, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:65kDa.Observed band size:65kDa.