Anti-Src抗体

Anti-Src抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310041Anti-Src(Phospho-Tyr529) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC60kDa

Sensitivity  The antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.

Anti-Src抗体Immunogen  Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.

Host  Rabbit

Clone Number  

Anti-Src抗体Antibody type  Polyclonal antibody

Purified method  

Isotype  Rabbit IgG

Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Anti-Src抗体Dilution

WB~~1:500-1:1000

ICC~~1:100-1:200

IHC~~1:50-1:100

Western blot analysis of extracts from COS7 and 3T3 cells, using SRC(Phospho-Tyr529) Rabbit mAb.The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.

Immunofluorescence staining of methanol-fixed Hela cells using Src (Phospho-Tyr529) Antibody .

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529) Antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529) Antibody .