Anti-PTEN抗体

Anti-PTEN抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310023Anti-PTEN(Phospho-Ser380/Thr382/Thr383) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC54

Sensitivity  The antibody detects endogenous level of PTEN only when phosphorylated at serine 380 and threonine 382/383.

Anti-PTEN抗体Immunogen  Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from Human PTEN.

Host  Rabbit

Clone Number  

Antibody type  Polyclonal antibody

Purified method  

Anti-PTEN抗体Isotype  IgG

Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Dilution

WB~~1:500-1:1000

ICC~~1:100-1:200

Anti-PTEN抗体IHC~~1:50-1:100

Western blot analysis of extracts from Hela and C6 cells, treated with EGF or PMA, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .

Immunofluorescence staining of methanol-fixed Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .

Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence staining of methanol-fixed MEF cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .

Western blot detection of PTEN(Phospho-Ser380/Thr382/Thr383) in A549 cells untreated or treated with Alkaline phosphatase using PTEN(Phospho-Ser380/Thr382/Thr383) Rabbit pAb (dilution 1:1000, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:54kDa.Observed band size:54kDa.