Anti-Histone H2A.X抗体

Anti-Histone H2A.X抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

201082-2A9Anti-Histone H2A.X(Phospho-Ser139) Mouse mAbMouse mAbHuman,MouseWB,IF15

Sensitivity  This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.

Immunogen  Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.

Anti-Histone H2A.X抗体Host  Mouse

Clone Number  2A9-B5

Antibody type  Monoclonal antibody

Purified method  Affinity purified

Isotype  IgG2a

Anti-Histone H2A.X抗体Formulation  Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Dilution

WB~~1:2000

ICC~~1:400

Anti-Histone H2A.X抗体Other~~1:400

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper,untreated or Hydroxyurea-treated) and Hela(lower,untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X(Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or β-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.