Anti-GAPDH抗体

Anti-GAPDH抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

200306Anti-GAPDH(Human specific) Mouse mAbMouse mAbHuman,MonkeyWB37

Sensitivity  This antibody detects endogenous levels of human GAPDH and does not cross-react with related proteins.

Immunogen  Purified recombinant human GAPDH protein fragments expressed in E.coli.

Host  Mouse

Clone Number  3C3-A2-E10

Anti-GAPDH抗体Antibody type  Monoclonal antibody

Purified method  Affinity purified

Isotype  IgG1

Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.

Dilution

Anti-GAPDH抗体WB~~1:5000

Western blot detection of GAPDH(human specific) in SW480,A431,K562,Jurkat and Hela cell lysates using GAPDH(human specific) mouse mAb (1:5000 diluted). Predicted band size:37KDa.Observed band size:37KDa.

Anti-GAPDH抗体Western blot detection of GAPDH(human specific) in Jurkat,COS7,K562 and Hela cell lysates using GAPDH(human specific) mouse mAb (1:3000 diluted).Predicted band size:37KDa.Observed band size:37KDa.

Western blot detection of GAPDH(human specific) stability in K562 cell lysate(A：Stored at room temperature for 72 hours;B：Stored at -20℃) using GAPDH(human specific) mouse mAb (1:10000 diluted).Predicted band size:37KDa.Observed band size:37KDa.